Journal: Science Advances

Article Title: Discovery of diverse and high-quality mRNA capping enzymes through a language model–enabled platform

doi: 10.1126/sciadv.adt0402

Figure Lengend Snippet: ( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two CEs (MRV_5 and MRV_6) found in this work compared with commercial vaccinia CE (VCE) (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.

Article Snippet: The membrane was incubated overnight at 4°C with T7 RNAP (Proteintech, 29943-1-AP; 1:2000) or β-actin antibody (Abclonal, AC004; 1:5000).

Techniques: In Vivo, Activity Assay, Negative Control, Positive Control, Fluorescence, In Vitro, Control, Molecular Weight, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Upregulation of p16 INK4A in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma

doi: 10.31557/APJCP.2022.23.11.3753

Figure Lengend Snippet: (a) Immunofluorescence staining of CD3+ peripheral immune cells showing DAPI staining (i), p16 INK4A staining (ii), CD3 staining (iii) and merged image (iv), (b) Immunofluorescence staining of CD14+ peripheral immune cells showing DAPI staining (i), p16 INK4A staining (ii), CD3 staining (iii) and merged image (iv), (c) Scatter plot of p16 INK4A MFI of healthy controls and p16 INK4A MFI of CRC group in CD3+ and CD14+ cells. The bar on the scatter plot represents mean value

Article Snippet: Anti-CD3 primary mouse antibody (Cell signaling) and anti-CD14 primary mouse antibody (Cell signaling) were diluted 1:500.

Techniques: Immunofluorescence, Staining

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Upregulation of p16 INK4A in Peripheral White Blood Cells as a Novel Screening Marker for Colorectal Carcinoma

doi: 10.31557/APJCP.2022.23.11.3753

Figure Lengend Snippet: (a) Comparison of p16INK4A positive cells in white blood cells, CD3+ cells and CD14+ cells between CRC group and healthy control group, (b) Correlation of p16 INK4A -positive cells percentage and p16 INK4A mean fluorescent intensity of CRC patients and healthy controls in white blood cells, (c) Correlation of p16 INK4A -positive cells percentage and p16 INK4A mean fluorescent intensity of CRC patients and healthy controls in CD3+ cells, (d) Correlation of p16 INK4A -positive cells percentage and p16 INK4A mean fluorescent intensity of CRC patients and healthy controls in CD14+ cells

Article Snippet: Anti-CD3 primary mouse antibody (Cell signaling) and anti-CD14 primary mouse antibody (Cell signaling) were diluted 1:500.

Techniques: Comparison, Control